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EXPRESSION OF GAP JUNCTION CONNEXIN36 IN ADULT RAT RETINAL GANGLION CELLS

2002; Imperial College Press; Volume: 01; Issue: 01 Linguagem: Inglês

10.1142/s0219635202000025

1757-448X

Soh Hidaka, Toshiaki Kato, Ei‐ichi Miyachi,

Neuroscience and Neuropharmacology Research

Journal of Integrative NeuroscienceVol. 01, No. 01, pp. 3-22 (2002) Short CommunicationsNo AccessEXPRESSION OF GAP JUNCTION CONNEXIN36 IN ADULT RAT RETINAL GANGLION CELLSSOH HIDAKA, TOSHIAKI KATO, and EI-ICHI MIYACHISOH HIDAKADepartment of Physiology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan, TOSHIAKI KATODepartment of Physiology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan, and EI-ICHI MIYACHIDepartment of Physiology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japanhttps://doi.org/10.1142/S0219635202000025Cited by:24 PreviousNext AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsRecommend to Library ShareShare onFacebookTwitterLinked InRedditEmail AbstractElectrophysiological and ultrastructural studies have demonstrated that gap junctions connect diverse types of neurons in the central nervous system, permitting direct electrical and metabolic coupling. A member of gap junction channel subunit connexin36 (Cx36), is probed for the location of cell-to-cell communication in the mammalian retina, where gap junction networks of major classes of neurons are present. We present an analysis of the expression and localization of Cx36 protein in adult Wistar rat retina, using a newly generated polyclonal antibody against a sequence in the predicted cytoplasmic loop of the Cx36 amino acid alignment, deduced from the cDNA sequence. The affinity-purified antibody, recognizing a single 36-kDa protein, consistently labeled discrete puncta of subcellular structures likely to be associated with gap junctions in the inner plexiform layer, and also cytoplasm within somata and dendrites of retinal amacrine and ganglion cells, following examination with various fixation protocols and double labeling immuno-fluorescence. These results provide that prominent cell-to-cell communication appears in mature excitatory neurons such as retinal ganglion cells, in addition to inhibitory amacrine cells, mediated by gap junctions in the adult retina.Keywords:Connexingap junctionelectrical synapseexcitatory neuronretinal ganglion cellinhibitory amacrine cellcell-to-cell communicationanti-connexin36 antibodyimmunolabelingrat retina FiguresReferencesRelatedDetailsCited By 24Analytical methods for assessing retinal cell coupling using cut-loadingWilliam E. Myles, Sally A. McFadden and Alexandre Hiroaki Kihara19 July 2022 | PLOS ONE, Vol. 17, No. 7The Role of GJD2 (Cx36) in Refractive Error DevelopmentEmilie van der Sande, Annechien E. G. Haarman, Wim H. Quint, Kirke C. D. Tadema and Magda A. Meester-Smoor et al.9 Mar 2022 | Investigative Opthalmology & Visual Science, Vol. 63, No. 3Loss of Gap Junction Delta-2 (GJD2) gene orthologs leads to refractive error in zebrafishWim H. Quint, Kirke C. D. 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Arshavsky5 September 2014Gap junctional coupling in the vertebrate retina: Variations on one theme?Béla Völgyi, Tamás Kovács-Öller, Tamás Atlasz, Márta Wilhelm and Róbert Gábriel1 May 2013 | Progress in Retinal and Eye Research, Vol. 34Expression and modulation of connexin30.2, a novel gap junction protein in the mouse retinaLUIS PÉREZ DE SEVILLA MÜLLER, KARIN DEDEK, ULRIKE JANSSEN-BIENHOLD, ARNDT MEYER and MARIA M. KREUZBERG et al.11 June 2010 | Visual Neuroscience, Vol. 27, No. 3-4Dopamine-Stimulated Dephosphorylation of Connexin 36 Mediates AII Amacrine Cell UncouplingW. Wade Kothmann, Stephen C. Massey and John O'Brien25 November 2009 | The Journal of Neuroscience, Vol. 29, No. 47Dopaminergic modulation of tracer coupling in a ganglion-amacrine cell networkSTEPHEN L. MILLS, XIAO-BO XIA, HIDEO HOSHI, SALLY I. FIRTH and MARGARET E. RICE et al.22 August 2007 | Visual Neuroscience, Vol. 24, No. 4Abundance and ultrastructural diversity of neuronal gap junctions in the OFF and ON sublaminae of the inner plexiform layer of rat and mouse retinaN. Kamasawa, C.S. Furman, K.G.V. Davidson, J.A. Sampson and A.R. Magnie et al.1 Nov 2006 | Neuroscience, Vol. 142, No. 4What is hidden in the pannexin treasure trove: the sneak peek and the guessworkOxana Litvin, Anya Tiunova, Yvette Connell-Alberts, Yuri Panchin and Ancha Baranova1 Jul 2006 | Journal of Cellular and Molecular Medicine, Vol. 10, No. 3Association of connexin36 and zonula occludens-1 with zonula occludens-2 and the transcription factor zonula occludens-1-associated nucleic acid-binding protein at neuronal gap junctions in rodent retinaC. Ciolofan, X.-B. Li, C. Olson, N. Kamasawa and B.R. Gebhardt et al.1 Jan 2006 | Neuroscience, Vol. 140, No. 2Expression and function of the neuronal gap junction protein connexin 36 in developing mammalian retinaKristi A. Hansen, Christine L. Torborg, Justin Elstrott and Marla B. Feller1 January 2005 | The Journal of Comparative Neurology, Vol. 493, No. 2STRUCTURAL AND FUNCTIONAL PROPERTIES OF HOMOLOGOUS ELECTRICAL SYNAPSES BETWEEN RETINAL AMACRINE CELLSSOH HIDAKA, TOSHIAKI KATO, and YOKO HASHIMOTO21 November 2011 | Journal of Integrative Neuroscience, Vol. 04, No. 03Signal compression in the sensory peripheryMauro Copelli, Rodrigo F. Oliveira, Antonio Carlos Roque and Osame Kinouchi1 Jun 2005 | Neurocomputing, Vol. 65-66Connexin36 mediates gap junctional coupling of alpha-ganglion cells in mouse retinaTimm Schubert, Joachim Degen, Klaus Willecke, Sheriar G. Hormuzdi and Hannah Monyer et al.1 January 2005 | The Journal of Comparative Neurology, Vol. 485, No. 3Intensity coding in two-dimensional excitable neural networksMauro Copelli and Osame Kinouchi1 Apr 2005 | Physica A: Statistical Mechanics and its Applications, Vol. 349, No. 3-4Dendrodendritic Electrical Synapses between Mammalian Retinal Ganglion CellsSoh Hidaka, Yasushi Akahori and Yoshikazu Kurosawa17 November 2004 | The Journal of Neuroscience, Vol. 24, No. 46Neuronal connexin36 association with zonula occludens-1 protein (ZO-1) in mouse brain and interaction with the first PDZ domain of ZO-1Xinbo Li, Carl Olson, Shijun Lu, Naomi Kamasawa and Thomas Yasumura et al.1 Apr 2004 | European Journal of Neuroscience, Vol. 19, No. 8 Recommended Vol. 01, No. 01 Metrics History Received 5 February 2002 Accepted 29 April 2002 KeywordsConnexingap junctionelectrical synapseexcitatory neuronretinal ganglion cellinhibitory amacrine cellcell-to-cell communicationanti-connexin36 antibodyimmunolabelingrat retinaPDF download