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Degumming of vegetable oils by a novel phospholipase B from Pseudomonas fluorescens BIT-18

2011; Elsevier BV; Volume: 102; Issue: 17 Linguagem: Inglês

10.1016/j.biortech.2011.05.050

1873-2976

Jiang FangYan, Jinmei Wang, Imdad Kaleem, Dazhang Dai, Xiaohong Zhou, Chun Li,

Microbial Metabolic Engineering and Bioproduction

Pseudomonas fluorescens BIT-18 was isolated from soil near a vegetable oil factory and shown to produce a B-type phospholipase. The enzyme was partially purified by ammonium sulfate precipitation. Gas chromatography demonstrated that the enzyme preparation hydrolyzed both the 1- and 2-ester bonds of phosphatidylcholine. When degumming of soybean, rapeseed, and peanut oil was performed with this enzyme preparation, oils with phosphorous contents lower than 5mg/kg were obtained after 5h of enzyme treatment at 40°C. The enzyme preparation did not show lipase activity, thus free fatty acids were only generated from the phospholipids. Therefore, this novel phospholipase B is potentially useful for the refining of high-quality oils with attractive yields.