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Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy

2009; Elsevier BV; Volume: 391; Issue: 1 Linguagem: Inglês

10.1016/j.ab.2009.05.005

1096-0309

Bong‐Hyun Jun, Mi Suk Noh, Gunsung Kim, Homan Kang, Jong-Ho Kim, Woo‐Jae Chung, Min‐Soo Kim, Yong-Kweon Kim, Myung‐Haing Cho, Dae Hong Jeong, Jeong Yong Lee,

Advanced biosensing and bioanalysis techniques

This article presents a prototype of a surface-enhanced Raman spectroscopy (SERS)-encoded magnetic bead of 8 μm diameter. The core part of the bead is composed of a magnetic nanoparticle (NP)-embedded sulfonated polystyrene bead. The outer part of the bead is embedded with Ag NPs on which labeling molecules generating specific SERS bands are adsorbed. A silica shell is fabricated for further bioconjugation and protection of SERS signaling. Benzenethiol, 4-mercaptotoluene, 2-naphthalenethiol, and 4-aminothiophenol are used as labeling molecules. The magnetic SERS beads are used as substrates for protein sensing and screening with easy handling. As a model application, streptavidin-bound magnetic SERS beads are used to illustrate selective separation in a flow cytometry system, and the screened beads are spectrally recognized by Raman spectroscopy. The proposed magnetic SERS beads are likely to be used as a versatile solid support for protein sensing and screening in multiple assay technology.