Methionine and Cystine Requirements of Rainbow Trout
1983; Volume: 45; Issue: 3 Linguagem: Inglês
10.1577/1548-8659(1983)45[139
ISSN1548-8640
AutoresGary L. Rumsey, Jimmy Page, Milton L. Scott,
Tópico(s)Reproductive biology and impacts on aquatic species
ResumoAbstract Abstract Methionine and cystine requirements of rainbow trout (Salmo gairdneri) were studied by supplementing a 35% protein semipurified basal diet with these amino acids at graded levels in a factorial design. In experiment 1 (initial average weight of fish, 1.5 g), methionirie levels were 0.30, 0.45, and 0.60% of the diet and cystine levels were 0.04, 0.15, 0.30, 0.45, and 0.60%. In experiment 2 (initial average weight of fish, 8.8 g), methionine levels were 0.55, 0.75, and 0.95% and cystine levels were 0.04, 0.08, 0.12, 0.16, 0.24, and 0.32%. Methionine requirement was estimated to be between 0.55 and 0.75% in the presence of adequate dietary cystine and the cystine requirement was about 0.30% with a diet marginally deficient in methionine. The requirement for both sulfur amino acids was, therefore, between 0.85 and 1.05% of the diet or 2.50 and 3.00% of the protein. Molar efficiency of conversion of dietary methionine to cystine was highest (80%) when the diet was deficient in methionine; this efficiency decreased as levels of dietary methionine increased. Bilateral cataracts occurred in methionine-deficient but not in cystine-deficient trout.
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