Escherichia coli extract-catalyzed recombination in switch regions of mouse immunoglobulin genes.
1983; National Academy of Sciences; Volume: 80; Issue: 9 Linguagem: Inglês
10.1073/pnas.80.9.2666
ISSN1091-6490
AutoresTohru Kataoka, Shunichi Takeda, T Honjo,
Tópico(s)Bacterial Genetics and Biotechnology
ResumoWe have shown that Escherichia coli extracts catalyze recombination between mouse immunoglobulin mu and alpha genes inserted separately in lambda phage vectors carrying different genetic markers. Most of the recombination sites in the inserts are located in the switch regions of the heavy chain genes, as previously found in the expressed genes of myeloma cells. The recombination took place at relatively high frequency (10(-4)). The recombinational system in E. coli or lambda phage seems to prefer short nucleotide sequences similar to those used in the class switch recombination.
Referência(s)