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Genome sequence of Halobacterium species NRC-1

2000; National Academy of Sciences; Volume: 97; Issue: 22 Linguagem: Inglês

10.1073/pnas.190337797

1091-6490

Wailap Victor Ng, Sean P. Kennedy, Gregory G. Mahairas, Brian R. Berquist, Min Pan, Hem D. Shukla, Stephen R. Lasky, Nitin S. Baliga, Vésteinn Thórsson, Jennifer Sbrogna, Steven Swartzell, Douglas Weir, John C. Hall, Timothy Dahl, Russell Welti, Young Ah Goo, Brent Leithauser, Kim Keller, Randy Cruz, Michael J. Danson, David W. Hough, Deborah G. Maddocks, Peter E. Jablonski, Mark P. Krebs, Christine M. Angevine, Heather Dale, Thomas A. Isenbarger, Ronald F. Peck, Mechthild Pohlschröder, John L. Spudich, Kwang-Hwan Jung, M. Shahid Alam, Tracey Freitas, Shaobin Hou, Charles J. Daniels, Patrick P. Dennis, Arina D. Omer, H. Alexander Ebhardt, Todd M. Lowe, Ping Liang, Monica Riley, Leroy Hood, Shiladitya DasSarma,

Amino Acid Enzymes and Metabolism

We report the complete sequence of an extreme halophile, Halobacterium sp. NRC-1, harboring a dynamic 2,571,010-bp genome containing 91 insertion sequences representing 12 families and organized into a large chromosome and 2 related minichromosomes. The Halobacterium NRC-1 genome codes for 2,630 predicted proteins, 36% of which are unrelated to any previously reported. Analysis of the genome sequence shows the presence of pathways for uptake and utilization of amino acids, active sodium-proton antiporter and potassium uptake systems, sophisticated photosensory and signal transduction pathways, and DNA replication, transcription, and translation systems resembling more complex eukaryotic organisms. Whole proteome comparisons show the definite archaeal nature of this halophile with additional similarities to the Gram-positive Bacillus subtilis and other bacteria. The ease of culturing Halobacterium and the availability of methods for its genetic manipulation in the laboratory, including construction of gene knockouts and replacements, indicate this halophile can serve as an excellent model system among the archaea.