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Epstein - Barr virus episome-based promoter function in human myeloid cells

1989; Oxford University Press; Volume: 17; Issue: 5 Linguagem: Inglês

10.1093/nar/17.5.1989

1362-4962

Christoph Hauer, Robert R. Getty, Mark L. Tykocinski,

T-cell and Retrovirus Studies

Epstein-Barr virus (EBV) episomal replicons offer an expeditious means for amplifying transfected genes in human cells. A panel of EBV episomes was constructed to assess the relative utility of five distinct eukaryotic promoter elements for high level and inducible gene expression in stably transfected human myeloid leukemia cells. The Rous sarcoma virus 3′ long terminal repeat (LTR) was most highly suited for EBV episome-based gene expression, whereas the lymphopapilloma virus and the SV40 early regulatory elements exhibited substantially lower activities. Chemically responsive promoter elements, such as the SV40 early, human metallothionein II and rat GRP78 gene promoters, retained their inducibility when ÊBV episome-based. Differences in gene expression obtained with the episomes reflected differential promoter activity rather than significant variations in episome copy numbers per cell. These observations provide guidelines for the optimal design of EBV episomal expression vectors for human expression work.