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Determinação de citrato de sildenafila e de tadalafila por cromatografia líquida de ultraeficiência com detecção por arranjo de diodos (CLUE-DAD)

2010; Brazilian Chemical Society; Volume: 33; Issue: 2 Linguagem: Inglês

10.1590/s0100-40422010000200028

ISSN

1678-7064

Autores

Rafael Scorsatto Ortiz, Marina Venzon Antunes, Rafael Linden,

Tópico(s)

Pharmacological Effects and Assays

Resumo

LIQUID CHROMATOGRAPHY WITH DIODE-ARRAY DETECTION (UPLC-DAD).A simple ultra-performance liquid chromatographic method for the simultaneous determination of sildenafil citrate and tadalafil was developed and validated.Sample preparation was dissolution in methanol, followed by centrifugation and dilution (1:10) with methanol.Analysis was performed in an Acquity ® UPLC system with Acquity ® BEH C18 column (2.1 x 50 mm, with 1.7 μm particles).The elution was isocratic with phosphate buffer pH 2.3 and acetonitrile (65:35, v/v) at a flow rate of 0.7 mL/min.The method presented adequate specificity, linearity, precision and accuracy and allowed the determination of the drugs in seized forensic samples.

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