A multiple adapter for the SW 25 rotor
1973; Elsevier BV; Volume: 55; Issue: 1 Linguagem: Inglês
10.1016/0003-2697(73)90318-7
ISSN1096-0309
AutoresMarie-Christine Guérin, D.H. Háyes,
Tópico(s)Bacterial Genetics and Biotechnology
ResumoAn easily made adapter whose use increases threefold the analytical capacity of the Spinco SW 25 rotor is described. Two types of commercially available polypropylene tube can be used with the adapters. The SW 25 rotor can be loaded with three adapters carrying in all nine 4.8 ml tubes with 4.4 ml sucrose gradients or with a combination of adapters and standard 34 ml SW 25 cellulose nitrate tubes containing 25 ml gradients. Resolution of total E. coli RNA in the 4.4 ml (50–100 μg RNA) and 25 ml (500–1000 μg RNA) gradients is equivalent.
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