Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Structural characterization of the cell wall d-glucans isolated from the mycelium of Botryosphaeria rhodina MAMB-05

2008; Elsevier BV; Volume: 343; Issue: 4 Linguagem: Inglês

10.1016/j.carres.2007.12.021

1873-426X

Maria de Lourdes Corradi da Silva, Eliane Kaori Fukuda, Ana Flora Dalberto Vasconcelos, Robert F. H. Dekker, Andreza Cândido Matias, Nilson K. Monteiro, Marilsa S. Cardoso, Aneli M. Barbosa, Joana Léa Meira Silveira, Guilherme L. Sassaki, Elaine R. Carbonero,

Microbial Metabolites in Food Biotechnology

Three d-glucans were isolated from the mycelium of the fungus Botryosphaeria rhodina MAMB-05 by sequential extraction with hot-water and hot aqueous KOH (2% w/v) followed by ethanol precipitation. Following their purification by gel permeation chromatography on Sepharose CL-4B, the structural characteristics of the d-glucans were determined by FT-IR and 13C NMR spectroscopy and, after methylation, by GC–MS. The hot-water extract produced a fraction designated Q1A that was a β-(1→6)-d-glucan with the following structure: The alkaline extract, when subjected to repeated freeze–thawing, yielded two fractions: K1P (insoluble) that comprised a β-(1→3)-d-glucan with β-d-glucose branches at C-6 with the structure: and K1SA (soluble) consisting of a backbone chain of α-(1→4)-linked d-glucopyranosyl residues substituted at O-6 with α-d-glucopyranosyl residues: