Indirect Laser-Induced Fluorescence Detection for Capillary Electrophoresis Using a Violet Diode Laser
2001; American Chemical Society; Volume: 73; Issue: 8 Linguagem: Inglês
10.1021/ac001301u
ISSN1520-6882
AutoresJeremy E. Melanson, Camille A. Boulet, Charles A. Lucy,
Tópico(s)Electrochemical sensors and biosensors
ResumoThe violet (415 nm) diode laser is used for indirect laser-induced fluorescence detection in capillary electrophoretic separations of inorganic anions and chemical warfare agent degradation products. Inorganic anions were detected using 8-hydroxypyrene-1,3,6-trisulfonic acid as the indirect probe and achieved submicromolar (40−80 ppb) detection limits in a 2-min separation. The chemical warfare agent degradation products methylphosphonic acid, ethyl methylphosphonate, isopropyl methylphosphonate, and pinacolyl methylphosphonate were detected using the porphyrin tetrakis(4-sulfophenyl)porphine as the indirect probe and achieved detection limits of 0.1 μM (9 ppb), which are 1 order of magnitude better than that achieved using indirect UV detection. Baseline stability achieved with the violet diode laser was excellent, with dynamic reserve (DR) values of >1000, which are 15 times better than that achieved using an unstabilized HeCd laser.
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