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Effects of 2 Concentrations of Sodium Citrate on Coagulation Test Results, von Willebrand Factor Concentration, and Platelet Function in Dogs

2007; Wiley; Volume: 21; Issue: 3 Linguagem: Inglês

10.1892/0891-6640(2007)21[472

1939-1676

F. Hernández Morales, C. Guillermo Couto, M.C. Iazbik,

Blood Coagulation and Thrombosis Mechanisms

Journal of Veterinary Internal MedicineVolume 21, Issue 3 p. 472-475 Open Access Effects of 2 Concentrations of Sodium Citrate on Coagulation Test Results, von Willebrand Factor Concentration, and Platelet Function in Dogs F. Morales, F. Morales Veterinary Teaching Hospital, The Ohio State University, Columbus OHSearch for more papers by this authorC.G. Couto DVM, Diplomate, ACVIM, C.G. Couto DVM, Diplomate, ACVIM Veterinary Teaching Hospital, The Ohio State University, Columbus OH Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus OH Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus OH. 601 Vernon L. Tharp Street, Columbus, OH 43210–1089; e-mail: [email protected].Search for more papers by this authorM.C. Iazbik, M.C. Iazbik Veterinary Teaching Hospital, The Ohio State University, Columbus OHSearch for more papers by this author F. Morales, F. Morales Veterinary Teaching Hospital, The Ohio State University, Columbus OHSearch for more papers by this authorC.G. Couto DVM, Diplomate, ACVIM, C.G. Couto DVM, Diplomate, ACVIM Veterinary Teaching Hospital, The Ohio State University, Columbus OH Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus OH Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus OH. 601 Vernon L. Tharp Street, Columbus, OH 43210–1089; e-mail: [email protected].Search for more papers by this authorM.C. Iazbik, M.C. Iazbik Veterinary Teaching Hospital, The Ohio State University, Columbus OHSearch for more papers by this author First published: 05 February 2008 https://doi.org/10.1111/j.1939-1676.2007.tb02992.xCitations: 23 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Background:Blood collection tubes containing 3.2% (0.109 M) sodium citrate, instead of 3.8% (0.129 M) sodium citrate, have recently become available in the United States. These tubes are visually indistinguishable from the traditional 3.8% sodium citrate tubes, except for wording on the label. Consequently, samples for hemostatic evaluation are frequently collected in tubes containing the lower concentration of sodium citrate. Hypothesis:Results of hemostasis assays are different in samples collected in 3.2% versus 3.8% sodium citrate. Animals:Twenty healthy dogs. Methods:This study aimed at determining whether results of standard coagulation tests, von Willebrand factor concentration (vWF:Ag), and platelet function with the platelet function analyzer PFA-100a were affected by the different concentrations of sodium citrate. Blood samples were collected in tubes containing either 3.2% or 3.8% sodium citrate concentrations and processed routinely for coagulation assays (one-stage prothrombin time [OSPT], activated partial thromboplastin time [aPTT], fibrinogen concentration, and platelet count), vWF:Ag, and platelet function assays with a PFA-100. Results:There was no significant difference between samples collected in 3.2% versus those collected in 3.8% sodium citrate for OSPT, aPTT, fibrinogen concentration, platelet count, or vWF:Ag. The closure times with collagen/adenosine diphosphate were significantly shorter (66 ± 8.1 versus 74.8 ± 9.7 seconds; P < .0001) with the 3.2% than with 3.8% sodium citrate concentration, and the hematocrit was significantly higher (47.9 ± 5.6 versus 46.0 ± 4.7 seconds; P= .03) in samples collected in 3.2% than in those collected in 3.8% sodium citrate. Conclusions and Clinical Importance: There is no clinically relevant effect of collection of blood into 3.2% or 3.8% sodium citrate. References 1 Couto CG. Disorders of hemostasis. In: R. Nelson, CG Couto, eds. Small Animal Internal Medicine, 3rd ed. St Louis , MO : Mosby; 2003: 1185–1199. Google Scholar 2 Jilma B. Platelet function analyzer (PFA-100): A tool to quantify congenital or acquired platelet dysfunction. 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