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Fucan effect on CHO cell proliferation and migration

2013; Elsevier BV; Volume: 98; Issue: 1 Linguagem: Inglês

10.1016/j.carbpol.2013.05.040

1879-1344

Leonardo Thiago Duarte Barreto Nobre, Arthur Anthunes Jacome Vidal, Jailma Almeida‐Lima, Ruth Medeiros Oliveira, Edgar Julian Paredes‐Gamero, Valquíria P. Medeiros, Edvaldo da Silva Trindade, Célia Regina Cavichiolo Franco, Helena B. Nader, Hugo Alexandre Oliveira Rocha,

Polysaccharides and Plant Cell Walls

Fucan is a term used to denominate sulfated l-fucose rich polysaccharides. Here, a heterofucan, named fucan B, was extracted from the Spatoglossum schröederi seaweed. This 21.5 kDa galactofucan inhibited CHO-K1 proliferation and migration when fibronectin was the substrate. Fucan B derivatives revealed that such effects depend on their degree of sulfation. Fucan B did not induce cell death, but promoted G1 cell cycle arrest. Western blotting and flow cytometry analysis suggest that fucan B binds to fibronectin and activates integrin, mainly integrin α5β1, which induces FAK/RAS/MEK/ERK activation. FAK activation inhibits CHO-K1 migration on fibronectin and ERK blocks cell cycle progression. This study indicates that fucan B could be applied in developing new antitumor drugs.