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The use of liquid polyacrylamide in electrophoresis: III. Properties of liquid polyacrylamide in the presence of cellulose acetate

1979; Elsevier BV; Volume: 92; Issue: 1 Linguagem: Inglês

10.1016/0003-2697(79)90630-4

1096-0309

Hans-Joachim Bode,

Nanopore and Nanochannel Transport Studies

Solutions of uncross-linked liquid polyacrylamide (LPA) stabilized by cellulose acetate are shown to represent efficient molecular sieves for RNA and for sodium dodecyl sulfate-protein complexes. Endosmosis and evaporation have to be considered to obtain corrected estimates of mobilities of the macro-ions. The efficiency of molecular sieving is strongly influenced by the chain length of the single polymer molecules. This can be shown both in terms of the steepness of the relationship between logarithm of molecular weight and mobility and in the relationship between log (KR) and log molecular weight. Comparable chain length effects are found in cross-linked polyacrylamide, suggesting a common mechanism of action of the two electrophoretic sieving media. The correlation of polymer viscosity and of the impedance towards migration of macro-ions, as demonstrated by LPA-electrophoresis, suggests the participation of hydrodynamic interactions in the molecular sieving process.