Portal de Periódicos da CAPES

Multiclass Analysis of Antibiotic Residues in Honey by Ultraperformance Liquid Chromatography−Tandem Mass Spectrometry

2009; American Chemical Society; Volume: 57; Issue: 5 Linguagem: Inglês

10.1021/jf8034572

1520-5118

José Luis Martı́nez Vidal, María del Mar Aguilera‐Luiz, Roberto Romero‐González, Antonia Garrido Frenich,

Antibiotics Pharmacokinetics and Efficacy

A method has been developed and validated for the simultaneous analysis of different veterinary drug residues (macrolides, tetracyclines, quinolones, and sulfonamides) in honey. Honey samples were dissolved with Na(2)EDTA, and veterinary residues were extracted from the supernatant by solid-phase extraction (SPE), using OASIS HLB cartridges. The separation and determination was carried out by ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS), using an electrospay ionization source (ESI) in positive mode. Data acquisition under MS/MS was achieved by applying multiple reaction monitoring (MRM) of two ion transitions per compound to provide a high degree of sensitivity and specificity. The method was validated, and mean recoveries were evaluated at three concentration levels (10, 50, and 100 microg/kg), ranging from 70 to 120% except for doxycycline, erythromycin, and tylmicosin with recovery higher than 50% at the three levels assayed. Relative standard deviations (RSDs) of the recoveries were less than 20% within the intraday precision and less than 25% within the interday precision. The limits of quantification (LOQs) were always lower than 4 microg/kg. The developed procedure was applied to 16 honey samples, and erythromycin, sarafloxacin, and tylosin were found in a few samples.