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BIBTEX RIS

PLGA Nanoparticles as Delivery Systems for Protoporphyrin IX in Topical PDT: Cutaneous Penetration of Photosensitizer Observed by Fluorescence Microscopy

2013; American Scientific Publishers; Volume: 13; Issue: 10 Linguagem: Inglês

10.1166/jnn.2013.7789

ISSN

1533-4899

Autores

Carolina Loureiro da Silva, José Orestes Del Ciampo, Fábia Cristina Rossetti, Maria Vitória Lopes Badra Bentley, Maria Bernadete Riemma Pierre,

Tópico(s)

Retinoids in leukemia and cellular processes

Resumo

Poly(D,L lactic-co-glycolic acid) (PLGA) based nanoparticles (NPs) are proposed for topical delivery of Protoporphyrin IX (PpIX) in Photodynamic Therapy of skin cancers. PpIX loaded into PLGA NPs showed nanometric average diameter (-280 nm), spherical forms and pH - 5.7, conditions suitable for topical application. In vitro release of PpIX from NPs was sustained up to 24 hr with a burst release effect of about 37.0% at 2 hr. Penetration and distribution of PpIX in hairless mice skin was determined by fluorescence microscopy 8 or 24 hrs after application of PpIX-NPs in the animals. At 24 hours, areas located in deeper regions of the skin were found to have greater fluorescence intensity. The finding indicates a localized effect of PpIX-NPs in the epidermis plus dermis--a site of action for topical PDT--and suggests a potential use of PpIX-NPs in PDT associated to skin cancer treatments.

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