The potential of carp to bioactivate benzo[a]pyrene to metabolites that bind to DNA
1991; Elsevier BV; Volume: 57; Issue: 3 Linguagem: Inglês
10.1016/0304-3835(91)90165-e
ISSN1872-7980
AutoresB. Kureleć, Sanja Krča, Ajay Garg, Ramesh C. Gupta,
Tópico(s)DNA and Nucleic Acid Chemistry
ResumoWe have investigated the formation of DNA adducts in starved, fed and 5,6-benzoflavone-pretreated carp following i.p. administration of benzo(a)pyrene. 32P-postlabeling analysis of the liver DNAs showed the presence of one predominant (>92%) adduct in all three groups. Cochromatography experiments revealed that the main adduct was identical to authentic BPDEI-dG (10β-(deoxyguanosin-N2-yl)-7β,8α,9α-trihydroxy-7,8,9,10 -tetrahydrobenzo[a]pyrene). The formation of the adduct was evident as early as 1.5 h post-treatment and the levels increased steadily up to 7 days, reaching about 125, 110 and 102 attomole/μg DNA in starved, fed and benzoflavone-pretreated carp, respectively. During this period, the benzo[a]pyrene-induced benzo[a]pyrene monooxygenase activity increased from the uninduced, natural level of about 3 pmol/mg per min to levels of 35, 62 and 79 pmol/mg per min in starving, fed and 5,6-benzoflavone pretreated fish, respectively. A slow but steady formation of the diolepoxide-dG adduct was also observed in the liver DNA of carp following p.o. treatment. These results indicate that carp can biotransform polycyclic aromatic hydrocarbons such as benzo[a]pyrene to ‘reactive’ metabolites that bind to DNA.
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