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Base changes in the fliC gene of Edwardsiella tarda: possible effects on flagellation and motility

2007; Inter-Research; Volume: 76; Linguagem: Inglês

10.3354/dao076113

1616-1580

J. Okuda, Futoshi Murayama, Eisuke Yamanoi, Erika Iwamoto, Satoshi Matsuoka, Mitsuaki Nishibuchi, Toshihiro Nakai,

Protist diversity and phylogeny

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 76:113-121 (2007) - doi:10.3354/dao076113 Base changes in the fliC gene of Edwardsiella tarda: possible effects on flagellation and motility Jun Okuda1,*, Fumiyasu Murayama1, Eisuke Yamanoi1, Emi Iwamoto1, Satoru Matsuoka2, Mitsuaki Nishibuchi3, Toshihiro Nakai1 1Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8528, Japan 2Ehime Prefectural Chuyo Fisheries Experimental Station, Iyo, Ehime 799-3125, Japan 3Center for Southeast Asian Studies, Kyoto University, Kyoto 606-8501, Japan *Email: okudaj@hiroshima-u.ac.jp ABSTRACT: Edwardsiella tarda is a broad host-range pathogen infecting both animals and humans. E. tarda isolates from red sea bream Pagrus major are non-motile, whereas isolates from Japanese eel Anguilla japonica and Japanese flounder Paralichthys olivaceus are motile with peritrichous flagella. We compared the fliC gene coding for flagellin (FliC) in motile and non-motile E. tarda strains isolated from diseased fish. Twenty-two amino acid residues differed in the predicted FliC amino acid sequences between non-motile and motile strains. There were no significant differences either in the upstream sequences regulating transcription of the fliC gene or in the fliC transcript levels between motile and non-motile strains. The predicted secondary structure of FliC in non-motile E. tarda differed from that of motile strains, and the modeled data suggested that the secondary structure may be the important factor responsible for non-flagellation in the non-motile strains. KEY WORDS: Edwardsiella tarda · fliC gene sequence · Base changes · Flagellation · Motility · Red sea bream · Japanese flounder Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 76, No. 2. Online publication date: June 29, 2007 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2007 Inter-Research.