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Coumarin-Derived Cu 2+ -Selective Fluorescence Sensor: Synthesis, Mechanisms, and Applications in Living Cells

2009; American Chemical Society; Volume: 131; Issue: 5 Linguagem: Inglês

10.1021/ja808611d

1943-2984

Hyo Sung Jung, Pil Seung Kwon, Jeong Won Lee, Jae Il Kim, Chang Seop Hong, Jong Wan Kim, Shihai Yan, Jin Yong Lee, Jung Hwa Lee, Taiha Joo, Jong Seung Kim,

Analytical Chemistry and Sensors

A novel coumarin-based fluorogenic probe bearing the 2-picolyl unit (1) was developed as a fluorescent chemosensor with high selectivity and suitable affinity in biological systems toward Cu2+ over other cations tested. The fluorescence on−off mechanism was studied by femtosecond time-resolved fluorescence (TRF) upconversion technique and ab initio calculations. The receptor can be applied to the monitoring of Cu2+ ion in aqueous solution with a pH span 4−10. To confirm the suitability of 1 for biological applications, we also employed it for the fluorescence detection of the changes of intracellular Cu2+ in cultured cells. The results indicate that 1 should be useful for the fluorescence microscopic imaging and the study on the biological functions of Cu2+.