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Superbeads: Immobilization in “Sweet” Chemistry

2003; Wiley; Volume: 9; Issue: 2 Linguagem: Inglês

10.1002/chem.200390038

1521-3765

Jozef Nahálka, Ziye Liu, Xi Chen, Peng George Wang,

Enzyme Production and Characterization

Abstract Enzymatic oligosaccharide synthesis using recombinant glycosyltransferases is able to overcome the difficulties associated with chemical methods. Nonetheless, sugar nucleotide regeneration cycles are necessary for the glycosylation. The multistep enzyme reaction can be efficiently carried out on superbeads that are prepared by immobilizing multienzyme mixtures on bead support through fused binding domains.