Differential regulation of a Thy-1 gene in transgenic mice.
1987; National Academy of Sciences; Volume: 84; Issue: 6 Linguagem: Inglês
10.1073/pnas.84.6.1492
ISSN1091-6490
AutoresGeorge Kollias, Eugenia Spanopoulou, Frank Grosveld, M. A. Ritter, J. N. Beech, Richard J. Morris,
Tópico(s)RNA and protein synthesis mechanisms
ResumoWe have generated Thy-1.1-transgenic Thy-1.2 mice to study the developmental expression of the Thy-1 gene in detail by transcriptional and immunological methods. In brain, the expression of the injected gene was identical to that of the endogenous gene in a tissue- and development-specific manner. In lymphoid tissue, the transferred gene was also expressed correctly in the early phases of T-cell lineage development; however, as the T cells matured, the transcription of the transferred gene, but not the endogenous gene, was suppressed. This result shows that different regulatory elements are used to express the Thy-1 gene in early and late lymphoid development.
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