Portal de Periódicos da CAPES

Wrong Primer for Rat Angiotensinogen mRNA

2005; American Physical Society; Volume: 288; Issue: 5 Linguagem: Dinamarquês

10.1152/ajprenal.00343.2004

1931-857X

Pisut Katavetin, Masaomi Nangaku, Toshiro Fujita,

Hormonal Regulation and Hypertension

LETTERS TO THE EDITORWrong Primer for Rat Angiotensinogen mRNAPisut Katavetin, Masaomi Nangaku, and Toshiro FujitaPisut Katavetin, Masaomi Nangaku, and Toshiro FujitaPublished Online:01 May 2005https://doi.org/10.1152/ajprenal.00343.2004MoreSectionsPDF (28 KB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInEmailWeChat Dear Editors,We recently attempted to measure mRNA expression in a series of rat cell lines using the primer for rat angiotensinogen mRNA (sense primer: 5-TCC CTG TCC TGT AAT CCC TC-3 and antisense primer: 5-GGC TGC TGC TCA TCA TTT AT-3) from the paper by Vidotti et al. (2) published in this journal, unfortunately without success.After checking the primer with the rat angiotensinogen mRNA sequence database (NM 134432), we were surprised to find that the sense primer was not designed against mRNA. It did, however, match the intron of the rat angiotensinogen gene ( L00094) reported as intron D in a previous study that analyzed the structural organization of the rat angiotensinogen gene (1).Theoretically, this pair of primers should not be able to amplify the part of the rat angiotensiogen mRNA sequence. It is therefore puzzling that their data showed successful amplification by PCR reaction with a product size of 398 base pairs.Given that Vidotti et al. (2) used this primer sequence in their study, we further question their finding that high glucose levels stimulate increased angiotensinogen expression in mesangial cells.Moreover, if they were able to obtain the PCR products with this pair on a consistent basis, we suggest the possibility of genomic DNA contamination of the RNA samples and therefore also question the quality of the RNA purification used.REFERENCES1 Tanaka T, Ohkubo H, and Nakanishi S. Common structural organization of the angiotensinogen and the α1-antitrypsin genes. J Biol Chem259 : 8063–8065, 1984.Crossref | PubMed | ISI | Google Scholar2 Vidotti DB, Casarini DE, Cristovam PC, Leite CA, Schor N, and Boim MA. High glucose concentration stimulates intracellular renin activity and angiotensin II generation in rat mesangial cells. Am J Physiol Renal Physiol286 : F1039–F1045, 2004.Link | ISI | Google Scholar Download PDF Previous Back to Top FiguresReferencesRelatedInformationCited ByIdentification of human gene research articles with wrongly identified nucleotide sequences12 January 2022 | Life Science Alliance, Vol. 5, No. 4The thin ret(raction) line: biomedical journal responses to incorrect non-targeting nucleotide sequence reagents in human gene knockdown publications28 February 2021 | Scientometrics, Vol. 126, No. 4Letter to the EditorMirian A. Boim1 May 2006 | American Journal of Physiology-Renal Physiology, Vol. 290, No. 5 More from this issue > Volume 288Issue 5May 2005Pages F1078-F1078 Copyright & PermissionsCopyright © 2005 the American Physiological Societyhttps://doi.org/10.1152/ajprenal.00343.2004PubMed15821251History Published online 1 May 2005 Published in print 1 May 2005 Metrics