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Isolation and characterization of microsatellite loci from Asparagus acutifolius (Liliaceae)

2003; Wiley; Volume: 3; Issue: 2 Linguagem: Inglês

10.1046/j.1471-8286.2003.00411.x

1471-8286

Serena Aceto, Maria Sica, Graziella Gamba, Stefania Montieri, Annarita Farina, Luciano Gaudio,

Plant Pathogens and Resistance

Abstract The isolation of molecular markers in Asparagus acutifolius , a wild edible plant species, is important to characterize local ecotypes that could be cultivated and preserved. We isolated and characterized polymorphic microsatellite loci from A. acutifolius by constructing and screening an enriched DNA library. Primer pairs were designed for 12 loci. Seven primer pairs worked well during amplification reactions and were tested on a wild population from Pontecagnano (SA), Italy. These loci showed a high level of genetic variability, with the numbers of alleles identified ranging from two to five and observed heterozygosity ranging from 0.20 to 0.73.