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Innovations in two-photon deep tissue microscopy

1999; IEEE Engineering in Medicine and Biology Society; Volume: 18; Issue: 5 Linguagem: Inglês

10.1109/51.790988

1937-4186

Ch. Buehler, Ki Hean Kim, Dong Chen, Barry R. Masters, Peter T. C. So,

Retinal Development and Disorders

The goal of this article is to provide a review of deep tissue studies based on two-photon microscopy. We will further explore three new developments that have significantly enhanced the power of two-photon imaging: (1) simultaneous two-photon fluorescence and confocal reflected-light imaging, (2) two-photon video-rate imaging, and (3) fluorescent spectroscopic measurements in deep tissue.