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Genetic diversity in Selenmonas ruminantium isolated from the rumen

1990; Oxford University Press; Volume: 73; Issue: 4 Linguagem: Inglês

10.1111/j.1574-6968.1990.tb03960.x

1574-6968

Harry J. Flint, Jacqueline Bisset,

Legume Nitrogen Fixing Symbiosis

Non-denaturing polyacrylamide gel electrophoresis was used to detect genetic variation at loci coding for there intracellular enzymes in the obligately anaerobic rumen bacterium Selenomonas ruminantium. Four mobility variants were detected for lactate dehydrigenase, seven for glucokinase and at least five for NADP-dependent glutamate dehydrogenase among 28 newly isolated, and eitght previously isolated strains from sheep and cattle. No evidence was found for an exclusive association of any particular electrophoretic mobility type with variable metabolic traits such as the ability to utilise lactate, to reduce nitrate or to ferment trehalose, sorbitol, rhamnose or glycerol. The most commonly occurring electromorph type was recovered from more than one animal, while most animals examined were show to harbour more than one electromorph type.