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1 H NMR Determination of lactate 13 C‐enrichment in skeletal muscle: Using a double quantum filter for the simultaneous editing of 13 C‐coupled and 13 C‐uncoupled methyl protons resonance

1995; Wiley; Volume: 34; Issue: 3 Linguagem: Inglês

10.1002/mrm.1910340312

1522-2594

Gilles Bloch, Laurence Jouvensal, Pierre G. Carlier,

Mitochondrial Function and Pathology

Abstract 1 H NMR simultaneous editing of 13 C‐coupled and 13 C‐uncoupled methyl protons resonance, using the selection of double quantum coherences by a gradient pulse, was analyzed in vitro and demonstrated in situ on the hindlimb of an exercised rat model postmortem. In vitro calibration showed agreement with theoretical analysis. High‐resolution NMR of muscle extract confirmed the accuracy of the lactate 13 C‐enrichment calculated using the in situ NMR data and the calibration factor obtained in vitro.