Vectors with restriction site banks V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites
1987; Elsevier BV; Volume: 51; Issue: 2-3 Linguagem: Inglês
10.1016/0378-1119(87)90316-7
ISSN1879-0038
AutoresJohn Davison, Michel Heusterspreute, Nathalie Chevalier, Vinh Ha-Thi, Françoise Brunei,
Tópico(s)Bacterial Genetics and Biotechnology
ResumoThe construction of a new wide-host-range, restriction-site bank, cosmid-cloning vehicle (pJRD215) is described. The wide-host-range properties and the ability to be transferred by conjugation, extend genetic engineering to those Gram-negative species that cannot be transformed. The vector permits the cloning of genes from Gram-negative bacteria using a complementation screening procedure in a mutant host. This procedure is simplified by the possibility of construction of a cosmid gene bank so that only a few hundred clones need to be screened. Subsequent subcloning of the gene of interest is facilitated by the presence of at least 23 unique cloning sites.
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