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Isolation and characterization of a heteropolysaccharide from the corm of Amorphophallus campanulatus

2009; Elsevier BV; Volume: 344; Issue: 18 Linguagem: Inglês

10.1016/j.carres.2009.09.025

1873-426X

Debsankar Das, Subhas Mondal, Sadhan K. Roy, Debabrata Maiti, Bibhas K. Bhunia, Tapas K. Maiti, Syed S. Islam,

Phytochemicals and Antioxidant Activities

A water-soluble polysaccharide isolated from the aqueous extract of the corm of Amorphophallus campanulatus was found to contain D-galactose, D-glucose, 4-O-acyl-D-methyl galacturonate, and l-arabinose in a molar ratio 2:1:1:1. Structural investigation of the polysaccharide was carried out using acid hydrolysis, methylation analysis, periodate oxidation study, and NMR studies ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above-mentioned experiments the structure of the repeating unit of the polysaccharide was established as: This molecule showed splenocyte activation.