Parallel confocal detection of single molecules in real time
2008; Optica Publishing Group; Volume: 33; Issue: 9 Linguagem: Inglês
10.1364/ol.33.001026
ISSN1539-4794
AutoresP. M. Lundquist, Cheng Zhong, Peiqian Zhao, Austin B. Tomaney, Paul Peluso, John W. Dixon, Brad Bettman, Y. Lacroix, Deborah P. Kwo, Etienne McCullough, Mark Maxham, Kevin Hester, Paul McNitt, Donald M. Grey, Carlos Henríquez‐Castillo, Mathieu Foquet, Stephen W. Turner, D. Zaccarin,
Tópico(s)Analytical Chemistry and Sensors
ResumoThe confocal detection principle is extended to a highly parallel optical system that continuously analyzes thousands of concurrent sample locations. This is achieved through the use of a holographic laser illumination multiplexer combined with a confocal pinhole array before a prism dispersive element used to provide spectroscopic information from each confocal volume. The system is demonstrated to detect and identify single fluorescent molecules from each of several thousand independent confocal volumes in real time.
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