
Extraction and concentration of biogenic calcium oxalate from plant leaves
2009; Sociedade Brasileira de Ciência do Solo; Volume: 33; Issue: 3 Linguagem: Inglês
10.1590/s0100-06832009000300025
ISSN1806-9657
AutoresLiovando Marciano da Costa, Jairo Tronto, Vera Regina Leopoldo Constantino, Marilane Kalyetta Almeida Fonseca, Alessandra Pacobahyba Oliveira, Marcelo Rocha da Costa,
Tópico(s)Mineralogy and Gemology Studies
ResumoThe objective of this study was to extract and concentrate calcium oxalate (CaOx) crystals from plant leaves that form the above mentioned crystals. The chemical and physical studies of CaOx from plant to be performed depend on an adequate amount of the crystals. The plant used in this study was croton (Codiaeum variegatum). The leaves were ground in a heavy duty blender and sieved through a 0.20 mm sieve. The suspension obtained was suspended in distilled water. The crystals were concentrated at the bottom of a test tube. The supernatant must be washed until it is free of plant pigments and other organic substances. Biogenic CaOx crystals have well-defined and sharp peaks, indicating very high crystallinity. Moreover, the CaOx crystals were not damaged during the extraction procedure, as can be seen on the scanning electron microscope images. The porposed method can be considered efficient to extract and concentrate biogenic calcium oxalate.
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