Sphingosine kinase 1 is required for migration, proliferation and survival of MCF‐7 human breast cancer cells
2005; Wiley; Volume: 579; Issue: 24 Linguagem: Inglês
10.1016/j.febslet.2005.08.055
ISSN1873-3468
AutoresSukumar Sarkar, Michael Maceyka, Nitai C. Hait, Steven W. Paugh, Heidi Sankala, Sheldon Milstien, Sarah Spiegel,
Tópico(s)Lipid Membrane Structure and Behavior
ResumoSphingosine‐1‐phosphate (S1P) is a potent lysolipid involved in a variety of biological responses important for cancer progression. Therefore, we investigated the role of sphingosine kinase type 1 (SphK1), the enzyme that makes S1P, in the motility, growth, and chemoresistance of MCF‐7 breast cancer cells. Epidermal growth factor (EGF), an important growth factor for breast cancer progression, activated and translocated SphK1 to plasma membrane. SphK1 was required for EGF‐directed motility. Downregulation of SphK1 in MCF‐7 cells reduced EGF‐ and serum‐stimulated growth and enhanced sensitivity to doxorubicin, a potent chemotherapeutic agent. These results suggest that SphK1 may be critical for growth, metastasis and chemoresistance of human breast cancers.
Referência(s)