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Mast Cell Chymase Regulates Dermal Mast Cell Number in Mice

2002; Elsevier BV; Volume: 290; Issue: 5 Linguagem: Inglês

10.1006/bbrc.2002.6365

1090-2104

Yoshiaki Tomimori, Tsuyoshi Muto, Harukazu Fukami, Kayo Saito, Chika Horikawa, Nobuo Tsuruoka, Kyoko Yamashiro, Masayuki Saito, Namino Sugiura, Motoo Sumida, Saki Kakutani, Yoshiaki Fukuda,

Asthma and respiratory diseases

Chymase inhibitor reduced the increase in the number of dermal mast cells in 2,4-dinitrofluorobenzene-induced dermatitis in a dose-dependent manner. Intradermal injection of human chymase to mouse ear significantly increased histamine content, the marker for mast cell number in the skin. These results suggest that chymase released by mast cells may participate in local mast cell accumulation in a positive feedback fashion. Immunohistochemical analysis revealed that the intradermal injection of chymase reduces expression of stem cell factor (SCF) on surface of the skin keratinocytes. In addition, incubation of human keratinocytes with chymase in vitro resulted in release of SCF into the culture medium. Since soluble SCF is thought to regulate mast cell number, the chymase-induced mast cell accumulation may occur via the ability of chymase to process membrane-bound SCF on the epidermal keratinocytes.