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FtmPT2, an N ‐Prenyltransferase from Aspergillus fumigatus , Catalyses the Last Step in the Biosynthesis of Fumitremorgin B

2008; Wiley; Volume: 9; Issue: 13 Linguagem: Inglês

10.1002/cbic.200800240

1439-7633

Alexander Grundmann, Т. А. Кузнецова, Shamil Sh. Afiyatullov, Shu‐Ming Li,

Fungal Biology and Applications

ChemBioChemVolume 9, Issue 13 p. 2059-2063 Communication FtmPT2, an N-Prenyltransferase from Aspergillus fumigatus, Catalyses the Last Step in the Biosynthesis of Fumitremorgin B Alexander Grundmann Dr., Alexander Grundmann Dr. Heinrich-Heine-Universität Düsseldorf, Institut für Pharmazeutische Biologie und Biotechnologie, Universitätsstrasse 1, 40225 Düsseldorf (Germany), Fax: (+49) 211-81-10617Search for more papers by this authorTatyana Kuznetsova Dr., Tatyana Kuznetsova Dr. Pacific Institute of Bioorganic Chemistry, Far-East Division, Russian Academy of Sciences pr. 100-letiya Vladivostoka, 159, 690022 Vladivostok (Russia)Search for more papers by this authorShamil Sh. Afiyatullov Dr., Shamil Sh. Afiyatullov Dr. Pacific Institute of Bioorganic Chemistry, Far-East Division, Russian Academy of Sciences pr. 100-letiya Vladivostoka, 159, 690022 Vladivostok (Russia)Search for more papers by this authorShu-Ming Li Prof. Dr., Shu-Ming Li Prof. Dr. [email protected] Heinrich-Heine-Universität Düsseldorf, Institut für Pharmazeutische Biologie und Biotechnologie, Universitätsstrasse 1, 40225 Düsseldorf (Germany), Fax: (+49) 211-81-10617Search for more papers by this author Alexander Grundmann Dr., Alexander Grundmann Dr. Heinrich-Heine-Universität Düsseldorf, Institut für Pharmazeutische Biologie und Biotechnologie, Universitätsstrasse 1, 40225 Düsseldorf (Germany), Fax: (+49) 211-81-10617Search for more papers by this authorTatyana Kuznetsova Dr., Tatyana Kuznetsova Dr. Pacific Institute of Bioorganic Chemistry, Far-East Division, Russian Academy of Sciences pr. 100-letiya Vladivostoka, 159, 690022 Vladivostok (Russia)Search for more papers by this authorShamil Sh. Afiyatullov Dr., Shamil Sh. Afiyatullov Dr. Pacific Institute of Bioorganic Chemistry, Far-East Division, Russian Academy of Sciences pr. 100-letiya Vladivostoka, 159, 690022 Vladivostok (Russia)Search for more papers by this authorShu-Ming Li Prof. Dr., Shu-Ming Li Prof. Dr. [email protected] Heinrich-Heine-Universität Düsseldorf, Institut für Pharmazeutische Biologie und Biotechnologie, Universitätsstrasse 1, 40225 Düsseldorf (Germany), Fax: (+49) 211-81-10617Search for more papers by this author First published: 27 August 2008 https://doi.org/10.1002/cbic.200800240Citations: 66Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract Last, but not least: The ftmPT2 gene, which was identified in a biosynthetic gene cluster for fumitremorgins in Aspergillus fumigatus, was over-expressed in Saccharomyces cerevisiae. The purified soluble dimeric His6–FtmPT2 was characterised biochemically. Structure elucidation of the enzymatic product showed that FtmPT2 catalyses the prenylation of 12,13-dihydroxyfumitremorgin C in the presence of dimethylallyl diphosphate at position N1 of the indole ring and so functions as an N-prenyltransferase (see scheme). Supporting Information Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2008/z800240_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. 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