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Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides

1985; Oxford University Press; Volume: 13; Issue: 1 Linguagem: Inglês

10.1093/nar/13.1.45

1362-4962

Tomas Kempe, Wesley I. Sundquist, Flora Chow, Shiu-Lok Hu,

Molecular Junctions and Nanostructures

Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5′-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-labeled oligonucleotide probes were studied.