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CD5-positive B cells in healthy elderly humans are a polyclonal B cell population

2000; Wiley; Volume: 30; Issue: 10 Linguagem: Inglês

10.1002/1521-4141(200010)30

1521-4141

Kathrin Geiger, Ulf Klein, Andreas Bräuninger, Stefan Berger, Korinna Leder, Klaus Rajewsky, Martin‐Leo Hansmann, Ralf Küppers,

T-cell and B-cell Immunology

European Journal of ImmunologyVolume 30, Issue 10 p. 2918-2923 Article CD5-positive B cells in healthy elderly humans are a polyclonal B cell population Kathrin D. Geiger, Kathrin D. Geiger Department of Pathology, University Hospital of the JWG-University Frankfurt, Frankfurt am Main, Germany Edinger-Institut, University Hospital of Frankfurt, Frankfurt am Main, GermanySearch for more papers by this authorUlf Klein, Ulf Klein Institute for Genetics, University of Cologne, Cologne, GermanySearch for more papers by this authorAndreas Bräuninger, Andreas Bräuninger Department of Pathology, University Hospital of the JWG-University Frankfurt, Frankfurt am Main, GermanySearch for more papers by this authorStefan Berger, Stefan Berger Department of Pathology, University Hospital of the JWG-University Frankfurt, Frankfurt am Main, GermanySearch for more papers by this authorKorinna Leder, Korinna Leder Department of Pathology, University Hospital of the JWG-University Frankfurt, Frankfurt am Main, GermanySearch for more papers by this authorKlaus Rajewsky, Klaus Rajewsky Institute for Genetics, University of Cologne, Cologne, GermanySearch for more papers by this authorMartin-Leo Hansmann, Martin-Leo Hansmann Department of Pathology, University Hospital of the JWG-University Frankfurt, Frankfurt am Main, GermanySearch for more papers by this authorRalf Küppers, Corresponding Author Ralf Küppers [email protected] Institute for Genetics, University of Cologne, Cologne, GermanyUniversity of Cologne, Department of Internal Medicine I, LFI E4 R706, Joseph-Stelzmannstr. 9, D-50931 Cologne, Germany Fax: +49-221-478-6383Search for more papers by this author Kathrin D. Geiger, Kathrin D. Geiger Department of Pathology, University Hospital of the JWG-University Frankfurt, Frankfurt am Main, Germany Edinger-Institut, University Hospital of Frankfurt, Frankfurt am Main, GermanySearch for more papers by this authorUlf Klein, Ulf Klein Institute for Genetics, University of Cologne, Cologne, GermanySearch for more papers by this authorAndreas Bräuninger, Andreas Bräuninger Department of Pathology, University Hospital of the JWG-University Frankfurt, Frankfurt am Main, GermanySearch for more papers by this authorStefan Berger, Stefan Berger Department of Pathology, University Hospital of the JWG-University Frankfurt, Frankfurt am Main, GermanySearch for more papers by this authorKorinna Leder, Korinna Leder Department of Pathology, University Hospital of the JWG-University Frankfurt, Frankfurt am Main, GermanySearch for more papers by this authorKlaus Rajewsky, Klaus Rajewsky Institute for Genetics, University of Cologne, Cologne, GermanySearch for more papers by this authorMartin-Leo Hansmann, Martin-Leo Hansmann Department of Pathology, University Hospital of the JWG-University Frankfurt, Frankfurt am Main, GermanySearch for more papers by this authorRalf Küppers, Corresponding Author Ralf Küppers [email protected] Institute for Genetics, University of Cologne, Cologne, GermanyUniversity of Cologne, Department of Internal Medicine I, LFI E4 R706, Joseph-Stelzmannstr. 9, D-50931 Cologne, Germany Fax: +49-221-478-6383Search for more papers by this author First published: 29 November 2000 https://doi.org/10.1002/1521-4141(200010)30:10 3.0.CO;2-CCitations: 20AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract B cell chronic lymphocytic leukemia (B-CLL) is a disease of the elderly and is characterized by a malignant clone of CD5+ B cells. In old mice, clonal expansions of CD5+ B cells are a common feature, and these animals frequently develop B-CLL. To investigate whether clonal expansion of CD5+ B cells also occurs in elderly humans, predisposing for the development of B-CLL, we analyzed VH gene rearrangements of CD5+ B cells from blood samples of four healthy, 65–82-years-old volunteers as markers of clonality. CD5+ and CD5– B cells were obtained by cell sorting, CDRIII of rearranged VH genes were amplified by polymerase chain reaction, and fragment length analysis was performed. All samples demonstrated a polyclonal pattern of VH gene length distribution. In addition, VH gene rearrangements were amplified and sequenced from sorted single cells of two of the donors. 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