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Regulation of Human Bone Marrow Leucopoiesis

1974; Wiley; Volume: 26; Issue: 2 Linguagem: Inglês

10.1111/j.1365-2141.1974.tb00468.x

1365-2141

David W. Golde, Martin J. Cline,

Hematological disorders and diagnostics

S ummary . The clonal growth of bone marrow in soft‐gel culture is largely dependent on the presence of colony‐stimulating factors (CSF). However, when human marrow is cultured in liquid medium with the in vitro diffusion chamber technique, proliferation and differentiation of granulocytes and mononuclear cells proceeds without a requirement for exogenously supplied CSF. The addition of highly active monocyte‐conditioned medium to the liquid marrow cultures did not result in a measurable change in viable or differential cell counts. Similarly, when mono‐cytes were co‐cultivated with bone marrow in a double‐chamber apparatus, no stimulation of marrow cell growth was observed. Colony‐stimulating activity was identified in conditioned medium derived from the liquid marrow cultures, and the cultured marrow cells themselves stimulated colony formation when incorporated as feeder layers. Retention of marrow cell responsiveness to CSF in liquid culture was demonstrated by cloning the cultured marrow cells in agar at intervals up to 2 weeks. Evidence was obtained that bone marrow cells of the monocyte‐macrophage line were primarily responsible for the elaboration of CSF in liquid culture. These data suggest a role for humoral interactions between haematopoietic cells within the marrow cavity.