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A prostaglandin oligomeric derivative inhibits activities of phospholipase and protease: A possible mechanism of membrane protection during ischemia

1989; Wiley; Volume: 7; Issue: 1 Linguagem: Inglês

10.1002/cbf.290070109

1099-0844

S. Tsuyoshi Ohnishi, Masayuki Katsuoka, Saburo Hidaka,

Bee Products Chemical Analysis

Abstract A prostaglandin oligomeric derivative was synthesized by alkaline treatment of prostaglandin E 1 . This compound protected the perfused rat heart from global ischemia. This compound was found to inhibit several lipolytic and proteolytic enzymes in vitro . When phospholipase A 2 from Naja naja venom was used as an enzyme and phosphatidylcholine was used as a substrate, 50 per cent inhibition was achieved at 50 μ M of the prostaglandin derivative. When trypsin and casein were used as enzyme and substrate, 50 per cent inhibition was obtained at 80 μ M . A possible mechanism of beneficial effect of this compound in protecting membranes during ischemia is discussed.