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Methods for the preparation of lysophosphatidylcholine

1971; Oxford University Press; Volume: 23; Issue: 9 Linguagem: Inglês

10.1111/j.2042-7158.1971.tb08746.x

2042-7158

Danuta S. Merryfield, J. J. Wren,

Lipid metabolism and biosynthesis

Abstract The surfactant and solubilizing properties of lysophosphatidylcholine (LPC) could have industrial uses if it became available in technical quantity and purity. The two most promising routes for obtaining it are isolation from cereal starches and mono-deacylation of egg phosphatidylcholine (PC). Baker's yeast autolysed by Letters's method yielded up to 0·3 % of LPC. The phospholipase A activity of pancreatin powder gave 80 % of the theoretical yield of LPC under optimum conditions; modifications designed to facilitate scale-up reduced the yield. Base-catalysed glycerolysis at 75° gave 23 % of the theoretical yield of LPC, and a reaction between PC and ethanolamine up to 45%. The crude product from ethanolamine after acetone precipitation gave an emulsifier containing LPC, PC and long-chain N-2-hydroxyethylamide.