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Direct determination of ciprofloxacin in admixtures with metronidazol and ampicillin by NMR

2005; Elsevier BV; Volume: 40; Issue: 2 Linguagem: Inglês

10.1016/j.jpba.2005.07.015

1873-264X

Uwe M. Reinscheid,

Analytical Chemistry and Chromatography

Methods to analyse mixtures of pharmaceutical compounds are often based on chromatographic separations coupled to UV detectors. These procedures typically have to verify with other methods that the specificity is sufficient because structural information is sometimes too limited. NMR methods can be particularly useful in the analysis of admixtures of antibiotics such as ciprofloxacin because of the inherent high selectivity. This can even be extended by the measurement of diffusion coefficients that help to identify the relevant signal for quantification. Taking advantage of the resolving power of NMR an analytical procedure to directly measure ciprofloxacin without separation steps is presented. The diffusion coefficients of the three components were measured. Tests of precision (repeatability) and recovery of ciprofloxacin without any separation steps in the presence of metronidazol and ampicillin were conducted.