Mouse Monoclonal IgM Antibody Against Human Lung Cancer Line SK-LC-3 with Specificity for H(O) Blood Group Antigen
1982; Mary Ann Liebert, Inc.; Volume: 1; Issue: 2 Linguagem: Inglês
10.1089/hyb.1.1982.1.139
ISSN2168-7897
AutoresB. Anger, Kenneth O. Lloyd, Herbert F. Oettgen, Lloyd J. Old,
Tópico(s)Blood groups and transfusion
ResumoSpleen cells from mice immunized with the human lung cancer line SK-LC-3 were fused with mouse NS-1 myeloma cells. One of the hybrid clones produced a monoclonal IgM antibody (designated F-3), detected with antimouse Ig-MHA and hemagglutination assays. This antibody was completely absorbed by O red cells and completely inhibited by low concentrations of H(O) glycoproteins and hog mucin (A + H). Bombay (Oh) red cells completely failed to absorb F-3 activity even after treatment with neuraminidase. A1, A2, A1,B, A2B, and B red cells and A- and B-glycoproteins were less effective in absorbing or inhibiting F-3 activity. Other glycoproteins (including those having Lea or blood group precursor structures) showed little or no inhibitory activity. Serum from nude mice carrying F-3 hybridoma agglutinated O and A2 red cells at a titer of 1:40,000 and 1:640, respectively. A1, A1B, A2B, and B red cells were agglutinated with titers of 1:80 or less. Monoclonal antibody F-3 is, therefore, highly specific for H(O) blood group determinants.
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