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Metabonomics Approach to Understanding Acute and Chronic Stress in Rat Models

2009; American Chemical Society; Volume: 8; Issue: 5 Linguagem: Inglês

10.1021/pr801086k

1535-3907

Xiaoyan Wang, Tie Zhao, Yunping Qiu, Mingming Su, Tao Jiang, Mingmei Zhou, Aihua Zhao, Jia Wang,

Stress Responses and Cortisol

The effects of acute and chronic stress on the production of systemic metabolites were investigated in male Sprague-Dawley (SD) rats. Metabolites excreted in urine were analyzed using GC/MS in conjunction with multivariate and univariate statistical techniques. SD rats were subjected to two kinds of acute stress and chronic unpredictable mild stress, respectively. Metabolic analysis demonstrated that urinary expression of a number of metabolites including glutamate, glutamine, homovanillate, proline, succinate, citrate, and tyrosine altered in the acute stress model in the same way as in the chronic model, while pimelate and hippurate changed in the opposite trend. The results suggested that the stress induced metabolic perturbations were reversible and nonspecific. Metabolic response to chronic combined stress revealed biochemical clues to depression-like symptoms validated by behavior and physiologic results. This study provides a noninvasive and dynamic analytical strategy for the characterization of endogenous metabolic perturbations induced by external stress.