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Genetic variability of Echinococcus granulosus based on the mitochondrial 16S ribosomal RNA gene

2013; Taylor & Francis; Volume: 26; Issue: 3 Linguagem: Inglês

10.3109/19401736.2013.840590

1940-1744

Ning Wang, Jiahai Wang, Dandan Hu, Xiuqin Zhong, Zhongrong Jiang, Aiguo Yang, Deng Shi-jin, Li Guo, Dawa Tsering, Shuxian Wang, Xiaobin Gu, Xuerong Peng, Guangyou Yang,

Parasitic Infections and Diagnostics

Echinococcus granulosus is the etiological agent of cystic echinococcosis, a major zoonotic disease of both humans and animals. In this study, we assessed genetic variability and genetic structure of E. granulosus in the Tibet plateau, using the complete mitochondrial 16 S ribosomal RNA gene for the first time. We collected and sequenced 62 isolates of E. granulosus from 3 populations in the Tibet plateau. A BLAST analysis indicated that 61 isolates belonged to E. granulosus sensu stricto (genotypes G1–G3), while one isolate belonged to E. canadensis (genotype G6). We detected 16 haplotypes with a haplotype network revealing a star-like expansion, with the most common haplotype occupying the center of the network. Haplotype diversity and nucleotide diversity were low, while negative values were observed for Tajima's D and Fu's Fs. AMOVA results and Fst values revealed that the three geographic populations were not genetically differentiated. Our results suggest that a population bottleneck or population expansion has occurred in the past, and that this explains the low genetic variability of E. granulosus in the Tibet Plateau.