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Immunoaffinity column clean-up techniques in food analysis: A review

2009; Elsevier BV; Volume: 878; Issue: 2 Linguagem: Inglês

10.1016/j.jchromb.2009.05.042

1873-376X

Hamide Z. Şenyuva, John Gilbert,

Mycotoxins in Agriculture and Food

This review provides a critical assessment of the applications of immunoaffinity columns for sample clean-up in the field of food safety. The performance of immunoaffinity columns are compared in terms of specificity, binding capacity and recovery, and commercial disposable columns are contrasted with home-made columns. Areas covered include multiple-use of columns, multiple-analyte columns, use with automated systems and validation of IAC methods. Publications illustrating the many varied applications of IAC for sample clean-up in the areas of mycotoxins, veterinary drug residues, pesticide residues, environmental contaminants and vitamins have been compiled, comparing extraction methods, achievable recovery, and illustrating the variety of end-detection methods that have been employed.