Role of the nuclear matrix in adenovirus maturation
1986; Elsevier BV; Volume: 4; Issue: 4 Linguagem: Inglês
10.1016/0168-1702(86)90085-7
ISSN1872-7492
AutoresG. Khittoo, Lyne Delorme, Claude V. Déry, Michel L. Treccmblay, Joseph Weber, Viviane Bibor‐Hardy, René Simard,
Tópico(s)RNA Interference and Gene Delivery
ResumoThe nuclear matrix has been implicated in several important cellular processes. In this paper, we investigate the role of the nuclear matrix in adenovirus type 2 assembly. Electron microscopic examination of nuclear matrices isolated from adenovirus infected Hep-2 cells clearly reveals that late in the lytic cycle, adenovirus capsids are intimately associated with the nuclear matrix. SDS-PAGE analysis showed that the viral core polypeptides V, PVII and 11 kDa were enriched in the nuclear matrix fraction. After a 3 h chase period a constant high ratio of PVII to VII prevailed in the nuclear matrix suggesting that mostly young virions and viral cores are bound to this structure. Most of the virus maturation endoproteinase activity co-purified with the nuclear matrix and the data suggest that the enzyme may be released from fragile young virions or assembly intermediates. Together these experiments suggest that the nuclear matrix is the site of adenovirus assembly and that mature virions may be released from the matrix by the viral endoproteinase.
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