The role of polyamines in interferon and retinoic acid mediated synergistic antiproliferative action
1989; Elsevier BV; Volume: 44; Issue: 1 Linguagem: Inglês
10.1016/0304-3835(89)90108-0
ISSN1872-7980
AutoresChristian Marth, P. Kirchebner, G. Daxenbichler,
Tópico(s)Toxoplasma gondii Research Studies
ResumoRetinoic acid alone has no effect on the human breast cancer cell line BT-20 but can amplify the antiproliferative action of interferon-gamma (IFN-gamma). In our system ornithine decarboxylase (ODC) activity correlates well with growth rate; it was investigated whether the antiproliferative effects of IFN-gamma and IFN-gamma plus retinoic acid could be attributed to suppression of ODC activity. The ODC inhibitor difluoromethyl-ornithine (DFMO), which is active as a single agent did not enhance growth inhibition induced by the biological response modifiers. The substitution of the BT-20 cells with putrescine, the product of the enzymatic reaction mediated by ODC, reversed DFMO induced antiproliferative action. On the other hand putrescine did not affect the proliferation of BT-20 cells treated with interferon alone or in combination with retinoic acid.
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