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Development of a Rapid Detection Procedure for Ctyptosporidium, Using In Vitro Cell Culture Combined with PCR

1996; Wiley; Volume: 43; Issue: 5 Linguagem: Inglês

10.1111/j.1550-7408.1996.tb05003.x

1550-7408

Paul A. Rochelle, Donna Ferguson, TROY J. HANDOJO, Ricardo León, Mic H. Stewart, Roy L. Wolfe,

Coccidia and coccidiosis research

SUMMARY A detection, viability, and infectivity assay was developed for Cryptosporidiurn parvum. Oocysts or excysted sporozoites were inoculated onto monolayers of CaCo‐2 cells grown on chamber slides. C. parvum infection was monitored by three methods: a) application of a fluorescein‐labeled anti‐sporozoite antibody; b) PCR of a heat‐shock protein gene fragment; and c) detection of mRNA from the heat‐shock protein gene by RT‐PCR.