Heterogeneity of immunoreactive dynorphin B - like material in human, rat, rabbit and guinea-pig heart
1991; Elsevier BV; Volume: 48; Issue: 6 Linguagem: Inglês
10.1016/0024-3205(91)90470-v
ISSN1879-0631
AutoresSanti Spampinato, Marco Canossa, Carlo Ventura, Tiziana Bachetti, Roberta Venturini, L Bastagli, P Bernardi, Sergio Solbes Ferri,
Tópico(s)Receptor Mechanisms and Signaling
ResumoImmunoreactive dynorphin B-like material (ir-dyn B) was detected in acetic acid extracts of human atrial specimens and of rat, rabbit and guinea-pig atria and ventricles by a validated radioimmunoassay. Levels were high in rabbit atrium (66.76 +/- 7.04 pmol/g) but lower and superimposable in human and rat atria (28.18 +/- 3.20 and 30.22 +/- 2.45 pmol/g, respectively). Gel permeation chromatography revealed ir-dyn B eluting close to column exclusion and in forms with an apparently higher molecular weight than authentic dyn B in human and rat samples. In contrast, almost all the immunoreactivity from rabbit and guinea-pig acetic extracts eluted as a single peak in the region of standard dyn B. Reverse-phase high performance liquid chromatography of the pooled gel chromatography fractions of this peak showed up a molecular form with the same retention time as authentic dyn B and a second minor peak of unknown immunoreactive material eluting three fractions earlier. Digestion with carboxypeptidase B excluded the hypothesis that this latter could be dyn B-Arg14. Therefore, it might be a metabolite of endogenous dyn B recognized by the antibody used in this study.
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