Determination of metronidazole, misonidazole and its metabolite in serum and urine on RP-18 high-performance thin-layer chromatographic plates
1981; Elsevier BV; Volume: 224; Issue: 3 Linguagem: Inglês
10.1016/s0378-4347(00)80220-0
ISSN1872-812X
AutoresE. Gattavecchia, Domenica Tonelli, A. Breccia,
Tópico(s)Ion channel regulation and function
ResumoA method for the determination of radiotherapeutic concentrations of metronidazole, misonidazole and its metabolite is described. The biological fluid (serum or urine) was deproteinized with acetone containing 2-nitroimidazole as internal standard, centrifuged and the supernatant evaporated under vacuum. The residue, dissolved in acetone, was applied to an HPTLC-RP-18 layer and, after development, quantitation was achieved with a scanning densitometer. The response was linear up to 180 μg/ml for all tested compounds and the detection limit was 0.5 μg/ml. Owing to its rapidity and sensitivity the method can be considered to be equivalent to high-performance liquid chromatography.
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