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Processed products of the hevein precursor in the latex of the rubber tree ( Hevea brasiliensis )

1995; Wiley; Volume: 363; Issue: 3 Linguagem: Inglês

10.1016/0014-5793(95)00309-w

1873-3468

Ukun M.S. Soedjanaatmadja, Toto Subroto, Jaap J. Beintema,

Natural product bioactivities and synthesis

The 20 kDa precursor of hevein and its C-terminal 14 kDa domain have been isolated. Sequence analysis of the C-terminal tryptic peptides of these proteins and comparison with the cDNA sequence indicate that they represent mature forms from which a C-terminal propeptide, possibly involved in vacuolar targeting, has been removed. The molar ratio of hevein to the C-terminal domain in the lutoid-body fraction of rubber latex is about 30:1. This indicates that not only the pre- and propeptides but also the 14 kDa domain are removed by proteolysis or other processes in the latex vessel after the processing of hevein has taken place.