The effect of fructose 1,6-diphosphate on pyruvate kinase from white muscle of three species of Mediterranean fish
1976; Elsevier BV; Volume: 55; Issue: 3 Linguagem: Inglês
10.1016/0305-0491(76)90319-9
ISSN0305-0491
AutoresJ.V. Bannister, Angela Xuereb‐Anastasi,
Tópico(s)Metabolomics and Mass Spectrometry Studies
ResumoAbstract 1. 1. The kinetics of pyruvate kinase in the absence and presence of fructose 1,6-diphosphate was investigated in preparations from white muscle of Boops boops (L.), Coryphaena hippurus (L.) and Mugil cephalus (L.). 2. 2. Mugil cephalus pyruvate kinase showed sigmoidal kinetics which changed to hyperbolic in the presence of fructose 1,6-diphosphate with increase in substrate affinity and maximum velocity. 3. 3. Coryphaena hippurus and Boops boops pyruvate kinase showed hyperbolic kinetics with increase in substrate affinity and maximum velocity in the presence of fructose 1,6-diphosphate, but the Boops boops enzyme was inhibited in the presence of the effector at phosphoenolpyruvate concentrations above 0.375 mM.
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